Comparison of fibrin adhesives prepared by 3 different methods

INTRODUCTION  Fibrin tissue adhesive, which has applications in several areas of medicine, can be prepared by different methods. AIM  To compare fibrin tissue adhesives prepared by 3 different methods. METHOD  In this prospective experimental laboratory study, fibrin tissue adhesives prepared by the use of plasma fibrinogen (group 1), cryoprecipitation (group 2), and precipitation by ammonium sulfate (group 3) were tested on 15 rabbits and 10 fragments of dura mater. The quality of the clots was assessed in terms of the success of the healing process, local toxicity, graft adhesion capacity, and degree of adhesion of 2 fragments of dura mater produced. RESULTS  All methods produced a clot with high adhesion and no toxicity, but tensile strength testing revealed that the glue produced from the ammonium sulfate-precipitated clot (group 3) was the strongest, requiring 39 g/cm(2) to separate the fragments as opposed to 23 g/cm(2) for group 2 and 13 g/cm(2) for group 1. CONCLUSION  All methods produced good results as far as clot formation and non-toxicity, but ammonium sulfate precipitation produced the best tensile strength and was thus the most effective method of preparing fibrin tissue adhesive.